This is a DNA hairpin equilibrium force (F1/2) calculator. You will need to input Temperature (ºC), Number of Nucleotides that consists hairpin, and ΔGfold (kcal/mol). Because ΔGfold (kcal/mol) depends on the temperature, if you change the temperature, please double-check if you have proper value. We often use IDT oligo calculator to obtain ΔGfold for hairpin of interest with experimental salt concentrations.
DNA Hairpin Equilibrium Force Calculator (F1/2)
If you find these tools useful in your work, we ask that you kindly acknowledge NIH support through the Center on Probes for Molecular Mechanotechnology (RM1GM145394), as well as reference the original research article (Woodside et al 2006, PNAS) in manuscripts and presentations.