This is a DNA hairpin equilibrium force (F1/2) calculator. You will need to input Temperature (ºC), Number of Nucleotides that consists hairpin, and ΔGfold (kcal/mol). Because ΔGfold (kcal/mol) depends on the temperature, if you change the temperature, please double-check if you have proper value. We often use IDT oligo calculator to obtain ΔGfold for hairpin of interest with experimental salt concentrations.