Collaborative Service Projects (CSPs)
As part of the CPMM effort to maximize dissemination and adoption of tension probe technology, we will provide direct access to probes and surfaces by offering reagents to CSP investigators. We will offer robust and hardened technologies to CSPs members, initially the DNA hairpin tension probes that are already used in the literature. Once the center is fully launched, we will start recruitment of new CBP members.
Available Technology
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Probe & Surface Technology
we envision that the CPMM probe and surface technology will be self-sustained through a fee structure. At that point in time, the TDP tools will be hardened and robust and easily disseminated to the mechanobiology community across the United States and also globally. The CPMM will support research in a model that matches support of CSPs. The CPMM will be sustained through a fee structure where reagents, substrates, and probes will be provided at a fee that reflects not only the cost of the raw reagents, but also the equipment service contracts, and staff salaries.
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Microscopy & Data Collection
The microscopy and data collection in the CPMM are for the most part not highly specialized and are available in many labs and core facilities. Indeed, a conventional epi-fluorescence microscope can be used to collect data, effectively eliminating any barrier. During the grant lifetime we will focus on development of protocols to facilitate adaptation of data collection, and these will be solidified for long term support. We will also work with our industry partners Nikon and ONI to integrate the specific imaging or analysis approaches into their software if appropriate.
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Data Analysis
we will create analysis protocols and software as described in the proposal. These will be disseminated using GitHub which will allow for wide adaptation. As the source code will be available to the community it will also be open for user-driven innovation.